Review

human colorectal carcinoma line hct 116 (ATCC)

ATCC is a verified supplier

ATCC manufactures this product

About

News

Press Release

Team

Advisors

Partners

Contact

Bioz Stars

Bioz vStars

Buy from Supplier

Structured Review

ATCC human colorectal carcinoma line hct 116

Immunohistochemical analysis of SLC35D3 expression in human tumors, normal tissues, and positive-control samples. (A) SLC35D3 staining in human tumors: (a) primary colon tumor; (b) corresponding lymph-node metastasis; (c) normal adjacent tissue (NAT) of a; (d) primary rectal tumor; (e) corresponding lymph-node metastasis; (f) NAT of d; (g–h) small-cell lung carcinoma (SCLC); (i) pancreatic neuroendocrine neoplasm; (j) pancreatic islet tumor. (B) SLC35D3 staining in human normal tissues: (a) cerebrum; (b) bone marrow; (c) lung; (d) heart; (e) liver; (f) kidney; (g) eye; (h) colon; (i) adrenal gland; (j) pancreas; (k) hypophysis; (l) stomach; (m) small intestine; (n) prostate. Arrowheads indicate SLC35D3-positive cells. (C) SLC35D3 staining of negative and positive control cell lines: (a) HCT 116-mock (negative control); (b) HCT 116-hSLC35D3 (engineered overexpression); (c) NCI–H1930 (endogenously SLC35D3-expressing).

Human Colorectal Carcinoma Line Hct 116, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 18026 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/human+colorectal+carcinoma+line+hct+116/HCT+116/pmc13096901-35-56-62
Average 99 stars, based on 18026 article reviews

human colorectal carcinoma line hct 116 - by Bioz Stars, 2026-10

99/100 stars

Images

1) Product Images from "Integrated transcriptomic and proteomic validation identifies SLC35D3 as a tumor-selective surface antigen for colorectal and neuroendocrine carcinomas"

Article Title: Integrated transcriptomic and proteomic validation identifies SLC35D3 as a tumor-selective surface antigen for colorectal and neuroendocrine carcinomas

Journal: Biochemistry and Biophysics Reports

doi: 10.1016/j.bbrep.2026.102587

Figure Legend Snippet: Immunohistochemical analysis of SLC35D3 expression in human tumors, normal tissues, and positive-control samples. (A) SLC35D3 staining in human tumors: (a) primary colon tumor; (b) corresponding lymph-node metastasis; (c) normal adjacent tissue (NAT) of a; (d) primary rectal tumor; (e) corresponding lymph-node metastasis; (f) NAT of d; (g–h) small-cell lung carcinoma (SCLC); (i) pancreatic neuroendocrine neoplasm; (j) pancreatic islet tumor. (B) SLC35D3 staining in human normal tissues: (a) cerebrum; (b) bone marrow; (c) lung; (d) heart; (e) liver; (f) kidney; (g) eye; (h) colon; (i) adrenal gland; (j) pancreas; (k) hypophysis; (l) stomach; (m) small intestine; (n) prostate. Arrowheads indicate SLC35D3-positive cells. (C) SLC35D3 staining of negative and positive control cell lines: (a) HCT 116-mock (negative control); (b) HCT 116-hSLC35D3 (engineered overexpression); (c) NCI–H1930 (endogenously SLC35D3-expressing).

Techniques Used: Immunohistochemical staining, Expressing, Positive Control, Staining, Negative Control, Over Expression

Validation of cell-surface SLC35D3 expression in cancer cell lines by flow cytometry and comparison with CCLE transcriptomic data. Flow cytometry histograms of cell-surface SLC35D3 staining in human cancer cell lines (HCT 116, LoVo, QGP-1, NCI–H1930, and SNU-16). For each cell line, the MFI ratio (anti-SLC35D3/isotype) and the corresponding mRNA expression level (log2[TPM+1]) from the CCLE are indicated below the histogram. HCT 116 served as a negative control and showed minimal surface staining, consistent with a CCLE value of log2[TPM+1] = 0.0.

Figure Legend Snippet: Validation of cell-surface SLC35D3 expression in cancer cell lines by flow cytometry and comparison with CCLE transcriptomic data. Flow cytometry histograms of cell-surface SLC35D3 staining in human cancer cell lines (HCT 116, LoVo, QGP-1, NCI–H1930, and SNU-16). For each cell line, the MFI ratio (anti-SLC35D3/isotype) and the corresponding mRNA expression level (log2[TPM+1]) from the CCLE are indicated below the histogram. HCT 116 served as a negative control and showed minimal surface staining, consistent with a CCLE value of log2[TPM+1] = 0.0.

Techniques Used: Biomarker Discovery, Expressing, Flow Cytometry, Comparison, Staining, Negative Control

Immunohistochemical staining:

Article Title: Integrated transcriptomic and proteomic validation identifies SLC35D3 as a tumor-selective surface antigen for colorectal and neuroendocrine carcinomas
Article Snippet: The human pancreatic islet cell carcinoma line QGP-1 (Japanese Collection of Research Bioresources Cell Bank, Osaka, Japan; Cat. No. JCRB0183), human small-cell lung carcinoma line NCI–H1930 (American Type Culture Collection (ATCC), Manassas, VA, USA; Cat. No. CRL-5906), human colorectal carcinoma line LoVo (ATCC; Cat. No. CCL-229), human gastric carcinoma line SNU-16 (ATCC; Cat. No. CRL-5974), and human colorectal carcinoma line HCT 116 (ATCC; Cat. No. CCL-247) were used.. QGP-1, NCI–H1930, HCT 116, and SNU-16 cells were maintained in RPMI-1640 medium supplemented with 10% heat-inactivated fetal bovine serum (FBS; 56 °C, 30 min) at 37 °C in a humidified atmosphere of 5% CO 2 .QGP-1, NCI–H1930, HCT 116, and SNU-16 cells were maintained in RPMI-1640 medium supplemented with 10% heat-inactivated fetal bovine serum (FBS; 56 °C, 30 min) at 37 °C in a..

Article Title: Integrated transcriptomic and proteomic validation identifies SLC35D3 as a tumor-selective surface antigen for colorectal and neuroendocrine carcinomas
Article Snippet: The human pancreatic islet cell carcinoma line QGP-1 (Japanese Collection of Research Bioresources Cell Bank, Osaka, Japan; Cat. No. JCRB0183), human small-cell lung carcinoma line NCI–H1930 (American Type Culture Collection (ATCC), Manassas, VA, USA; Cat. No. CRL5906), human colorectal carcinoma line LoVo (ATCC; Cat. No. CCL229), human gastric carcinoma line SNU-16 (ATCC; Cat. No. CRL5974), and human colorectal carcinoma line HCT 116 (ATCC; Cat. No. CCL-247) were used.. QGP-1, NCI–H1930, HCT 116, and SNU-16 cells were maintained in RPMI-1640 medium supplemented with 10% heatinactivated fetal bovine serum (FBS; 56 ◦C, 30 min) at 37 ◦C in a humidified atmosphere of 5% CO2.QGP-1, NCI–H1930, HCT 116, and SNU-16 cells were maintained in RPMI-1640 medium supplemented with 10% heatinactivated fetal bovine serum (FBS; 56 ◦C, 30 min) at 37 ◦C in a..

Expressing:

Article Title: Integrated transcriptomic and proteomic validation identifies SLC35D3 as a tumor-selective surface antigen for colorectal and neuroendocrine carcinomas
Article Snippet: The human pancreatic islet cell carcinoma line QGP-1 (Japanese Collection of Research Bioresources Cell Bank, Osaka, Japan; Cat. No. JCRB0183), human small-cell lung carcinoma line NCI–H1930 (American Type Culture Collection (ATCC), Manassas, VA, USA; Cat. No. CRL5906), human colorectal carcinoma line LoVo (ATCC; Cat. No. CCL229), human gastric carcinoma line SNU-16 (ATCC; Cat. No. CRL5974), and human colorectal carcinoma line HCT 116 (ATCC; Cat. No. CCL-247) were used.. QGP-1, NCI–H1930, HCT 116, and SNU-16 cells were maintained in RPMI-1640 medium supplemented with 10% heatinactivated fetal bovine serum (FBS; 56 ◦C, 30 min) at 37 ◦C in a humidified atmosphere of 5% CO2.QGP-1, NCI–H1930, HCT 116, and SNU-16 cells were maintained in RPMI-1640 medium supplemented with 10% heatinactivated fetal bovine serum (FBS; 56 ◦C, 30 min) at 37 ◦C in a..

Positive Control:

Article Title: Integrated transcriptomic and proteomic validation identifies SLC35D3 as a tumor-selective surface antigen for colorectal and neuroendocrine carcinomas
Article Snippet: The human pancreatic islet cell carcinoma line QGP-1 (Japanese Collection of Research Bioresources Cell Bank, Osaka, Japan; Cat. No. JCRB0183), human small-cell lung carcinoma line NCI–H1930 (American Type Culture Collection (ATCC), Manassas, VA, USA; Cat. No. CRL5906), human colorectal carcinoma line LoVo (ATCC; Cat. No. CCL229), human gastric carcinoma line SNU-16 (ATCC; Cat. No. CRL5974), and human colorectal carcinoma line HCT 116 (ATCC; Cat. No. CCL-247) were used.. QGP-1, NCI–H1930, HCT 116, and SNU-16 cells were maintained in RPMI-1640 medium supplemented with 10% heatinactivated fetal bovine serum (FBS; 56 ◦C, 30 min) at 37 ◦C in a humidified atmosphere of 5% CO2.QGP-1, NCI–H1930, HCT 116, and SNU-16 cells were maintained in RPMI-1640 medium supplemented with 10% heatinactivated fetal bovine serum (FBS; 56 ◦C, 30 min) at 37 ◦C in a..

Staining:

Article Title: Integrated transcriptomic and proteomic validation identifies SLC35D3 as a tumor-selective surface antigen for colorectal and neuroendocrine carcinomas
Article Snippet: The human pancreatic islet cell carcinoma line QGP-1 (Japanese Collection of Research Bioresources Cell Bank, Osaka, Japan; Cat. No. JCRB0183), human small-cell lung carcinoma line NCI–H1930 (American Type Culture Collection (ATCC), Manassas, VA, USA; Cat. No. CRL5906), human colorectal carcinoma line LoVo (ATCC; Cat. No. CCL229), human gastric carcinoma line SNU-16 (ATCC; Cat. No. CRL5974), and human colorectal carcinoma line HCT 116 (ATCC; Cat. No. CCL-247) were used.. QGP-1, NCI–H1930, HCT 116, and SNU-16 cells were maintained in RPMI-1640 medium supplemented with 10% heatinactivated fetal bovine serum (FBS; 56 ◦C, 30 min) at 37 ◦C in a humidified atmosphere of 5% CO2.QGP-1, NCI–H1930, HCT 116, and SNU-16 cells were maintained in RPMI-1640 medium supplemented with 10% heatinactivated fetal bovine serum (FBS; 56 ◦C, 30 min) at 37 ◦C in a..

Negative Control:

Article Title: Integrated transcriptomic and proteomic validation identifies SLC35D3 as a tumor-selective surface antigen for colorectal and neuroendocrine carcinomas
Article Snippet: The human pancreatic islet cell carcinoma line QGP-1 (Japanese Collection of Research Bioresources Cell Bank, Osaka, Japan; Cat. No. JCRB0183), human small-cell lung carcinoma line NCI–H1930 (American Type Culture Collection (ATCC), Manassas, VA, USA; Cat. No. CRL5906), human colorectal carcinoma line LoVo (ATCC; Cat. No. CCL229), human gastric carcinoma line SNU-16 (ATCC; Cat. No. CRL5974), and human colorectal carcinoma line HCT 116 (ATCC; Cat. No. CCL-247) were used.. QGP-1, NCI–H1930, HCT 116, and SNU-16 cells were maintained in RPMI-1640 medium supplemented with 10% heatinactivated fetal bovine serum (FBS; 56 ◦C, 30 min) at 37 ◦C in a humidified atmosphere of 5% CO2.QGP-1, NCI–H1930, HCT 116, and SNU-16 cells were maintained in RPMI-1640 medium supplemented with 10% heatinactivated fetal bovine serum (FBS; 56 ◦C, 30 min) at 37 ◦C in a..

Over Expression:

Article Title: Integrated transcriptomic and proteomic validation identifies SLC35D3 as a tumor-selective surface antigen for colorectal and neuroendocrine carcinomas
Article Snippet: The human pancreatic islet cell carcinoma line QGP-1 (Japanese Collection of Research Bioresources Cell Bank, Osaka, Japan; Cat. No. JCRB0183), human small-cell lung carcinoma line NCI–H1930 (American Type Culture Collection (ATCC), Manassas, VA, USA; Cat. No. CRL5906), human colorectal carcinoma line LoVo (ATCC; Cat. No. CCL229), human gastric carcinoma line SNU-16 (ATCC; Cat. No. CRL5974), and human colorectal carcinoma line HCT 116 (ATCC; Cat. No. CCL-247) were used.. QGP-1, NCI–H1930, HCT 116, and SNU-16 cells were maintained in RPMI-1640 medium supplemented with 10% heatinactivated fetal bovine serum (FBS; 56 ◦C, 30 min) at 37 ◦C in a humidified atmosphere of 5% CO2.QGP-1, NCI–H1930, HCT 116, and SNU-16 cells were maintained in RPMI-1640 medium supplemented with 10% heatinactivated fetal bovine serum (FBS; 56 ◦C, 30 min) at 37 ◦C in a..

Biomarker Discovery:

Article Title: Integrated transcriptomic and proteomic validation identifies SLC35D3 as a tumor-selective surface antigen for colorectal and neuroendocrine carcinomas
Article Snippet: The human pancreatic islet cell carcinoma line QGP-1 (Japanese Collection of Research Bioresources Cell Bank, Osaka, Japan; Cat. No. JCRB0183), human small-cell lung carcinoma line NCI–H1930 (American Type Culture Collection (ATCC), Manassas, VA, USA; Cat. No. CRL5906), human colorectal carcinoma line LoVo (ATCC; Cat. No. CCL229), human gastric carcinoma line SNU-16 (ATCC; Cat. No. CRL5974), and human colorectal carcinoma line HCT 116 (ATCC; Cat. No. CCL-247) were used.. QGP-1, NCI–H1930, HCT 116, and SNU-16 cells were maintained in RPMI-1640 medium supplemented with 10% heatinactivated fetal bovine serum (FBS; 56 ◦C, 30 min) at 37 ◦C in a humidified atmosphere of 5% CO2.QGP-1, NCI–H1930, HCT 116, and SNU-16 cells were maintained in RPMI-1640 medium supplemented with 10% heatinactivated fetal bovine serum (FBS; 56 ◦C, 30 min) at 37 ◦C in a..

Flow Cytometry:

Article Title: Integrated transcriptomic and proteomic validation identifies SLC35D3 as a tumor-selective surface antigen for colorectal and neuroendocrine carcinomas
Article Snippet: The human pancreatic islet cell carcinoma line QGP-1 (Japanese Collection of Research Bioresources Cell Bank, Osaka, Japan; Cat. No. JCRB0183), human small-cell lung carcinoma line NCI–H1930 (American Type Culture Collection (ATCC), Manassas, VA, USA; Cat. No. CRL5906), human colorectal carcinoma line LoVo (ATCC; Cat. No. CCL229), human gastric carcinoma line SNU-16 (ATCC; Cat. No. CRL5974), and human colorectal carcinoma line HCT 116 (ATCC; Cat. No. CCL-247) were used.. QGP-1, NCI–H1930, HCT 116, and SNU-16 cells were maintained in RPMI-1640 medium supplemented with 10% heatinactivated fetal bovine serum (FBS; 56 ◦C, 30 min) at 37 ◦C in a humidified atmosphere of 5% CO2.QGP-1, NCI–H1930, HCT 116, and SNU-16 cells were maintained in RPMI-1640 medium supplemented with 10% heatinactivated fetal bovine serum (FBS; 56 ◦C, 30 min) at 37 ◦C in a..

Comparison:

Article Title: Integrated transcriptomic and proteomic validation identifies SLC35D3 as a tumor-selective surface antigen for colorectal and neuroendocrine carcinomas
Article Snippet: The human pancreatic islet cell carcinoma line QGP-1 (Japanese Collection of Research Bioresources Cell Bank, Osaka, Japan; Cat. No. JCRB0183), human small-cell lung carcinoma line NCI–H1930 (American Type Culture Collection (ATCC), Manassas, VA, USA; Cat. No. CRL5906), human colorectal carcinoma line LoVo (ATCC; Cat. No. CCL229), human gastric carcinoma line SNU-16 (ATCC; Cat. No. CRL5974), and human colorectal carcinoma line HCT 116 (ATCC; Cat. No. CCL-247) were used.. QGP-1, NCI–H1930, HCT 116, and SNU-16 cells were maintained in RPMI-1640 medium supplemented with 10% heatinactivated fetal bovine serum (FBS; 56 ◦C, 30 min) at 37 ◦C in a humidified atmosphere of 5% CO2.QGP-1, NCI–H1930, HCT 116, and SNU-16 cells were maintained in RPMI-1640 medium supplemented with 10% heatinactivated fetal bovine serum (FBS; 56 ◦C, 30 min) at 37 ◦C in a..

Image Search Results

Journal: Biochemistry and Biophysics Reports

Article Title: Integrated transcriptomic and proteomic validation identifies SLC35D3 as a tumor-selective surface antigen for colorectal and neuroendocrine carcinomas

doi: 10.1016/j.bbrep.2026.102587

Figure Lengend Snippet: Immunohistochemical analysis of SLC35D3 expression in human tumors, normal tissues, and positive-control samples. (A) SLC35D3 staining in human tumors: (a) primary colon tumor; (b) corresponding lymph-node metastasis; (c) normal adjacent tissue (NAT) of a; (d) primary rectal tumor; (e) corresponding lymph-node metastasis; (f) NAT of d; (g–h) small-cell lung carcinoma (SCLC); (i) pancreatic neuroendocrine neoplasm; (j) pancreatic islet tumor. (B) SLC35D3 staining in human normal tissues: (a) cerebrum; (b) bone marrow; (c) lung; (d) heart; (e) liver; (f) kidney; (g) eye; (h) colon; (i) adrenal gland; (j) pancreas; (k) hypophysis; (l) stomach; (m) small intestine; (n) prostate. Arrowheads indicate SLC35D3-positive cells. (C) SLC35D3 staining of negative and positive control cell lines: (a) HCT 116-mock (negative control); (b) HCT 116-hSLC35D3 (engineered overexpression); (c) NCI–H1930 (endogenously SLC35D3-expressing).

Article Snippet: The human pancreatic islet cell carcinoma line QGP-1 (Japanese Collection of Research Bioresources Cell Bank, Osaka, Japan; Cat. No. JCRB0183), human small-cell lung carcinoma line NCI–H1930 (American Type Culture Collection (ATCC), Manassas, VA, USA; Cat. No. CRL-5906), human colorectal carcinoma line LoVo (ATCC; Cat. No. CCL-229), human gastric carcinoma line SNU-16 (ATCC; Cat. No. CRL-5974), and human colorectal carcinoma line HCT 116 (ATCC; Cat. No. CCL-247) were used.

Techniques: Immunohistochemical staining, Expressing, Positive Control, Staining, Negative Control, Over Expression

Journal: Biochemistry and Biophysics Reports

Article Title: Integrated transcriptomic and proteomic validation identifies SLC35D3 as a tumor-selective surface antigen for colorectal and neuroendocrine carcinomas

doi: 10.1016/j.bbrep.2026.102587

Figure Lengend Snippet: Validation of cell-surface SLC35D3 expression in cancer cell lines by flow cytometry and comparison with CCLE transcriptomic data. Flow cytometry histograms of cell-surface SLC35D3 staining in human cancer cell lines (HCT 116, LoVo, QGP-1, NCI–H1930, and SNU-16). For each cell line, the MFI ratio (anti-SLC35D3/isotype) and the corresponding mRNA expression level (log2[TPM+1]) from the CCLE are indicated below the histogram. HCT 116 served as a negative control and showed minimal surface staining, consistent with a CCLE value of log2[TPM+1] = 0.0.

Techniques: Biomarker Discovery, Expressing, Flow Cytometry, Comparison, Staining, Negative Control

10-HDA inhibited CRC cell growth and gap closure and increased intracellular ROS. ( A ) MTT analysis of HCT 116 and HT-29 cell viability after 48 h exposure to 10-HDA. ( B ) Colony formation of HCT 116 cells following 14 d treatment with 10-HDA. ( C ) Scratch assay analysis of HCT 116 gap closure after 24 h incubation with 10-HDA. ( D ) Flow cytometric analysis of HCT 116 intracellular ROS levels following 3 h exposure to 10-HDA. ** p < 0.01, *** p < 0.001, **** p < 0.0001; ns, no statistical significance. Control, vehicle-treated cells.

Journal: Foods

Article Title: 10-Hydroxy-2-decenoic Acid Suppresses Colorectal Cancer Progression by Inhibiting Wnt/β-Catenin Signaling and Promoting Apoptosis

doi: 10.3390/foods15091608

Figure Lengend Snippet: 10-HDA inhibited CRC cell growth and gap closure and increased intracellular ROS. ( A ) MTT analysis of HCT 116 and HT-29 cell viability after 48 h exposure to 10-HDA. ( B ) Colony formation of HCT 116 cells following 14 d treatment with 10-HDA. ( C ) Scratch assay analysis of HCT 116 gap closure after 24 h incubation with 10-HDA. ( D ) Flow cytometric analysis of HCT 116 intracellular ROS levels following 3 h exposure to 10-HDA. ** p < 0.01, *** p < 0.001, **** p < 0.0001; ns, no statistical significance. Control, vehicle-treated cells.

Article Snippet: Human colorectal carcinoma cell lines HCT 116 (ATCC; CCL-247) and HT-29 (ATCC; HTB-38) were purchased from Zhong Qiao Xin Zhou Biotechnology Co., Ltd. (Shanghai, China).

Techniques: Wound Healing Assay, Incubation, Control

10-HDA inhibited tumor growth and modified the histopathological features of HCT 116 xenografts in vivo. ( A ) Schematic overview of HCT 116 xenograft establishment and 10-HDA administration. ( B ) Representative images of xenograft tumors from saline-treated control mice and 10-HDA-treated mice, with corresponding tumor volume quantification. Control, blue; 100 mg/kg 10-HDA, orange; 200 mg/kg 10-HDA, purple. ( C ) H&E-stained sections of xenograft tumors. Red arrows indicate tumor cells; yellow arrows denote necrotic regions; blue arrows indicate atypical (pathological) mitotic figures; and green arrows indicate inflammatory cell infiltration. ( D ) Ki-67 immunohistochemical staining of xenograft tumors from control and 10-HDA-treated mice. n = 6; * p < 0.05, ** p < 0.01, **** p < 0.0001; ns, no statistical significance. Control, saline-treated tumor-bearing mice.

Journal: Foods

Article Title: 10-Hydroxy-2-decenoic Acid Suppresses Colorectal Cancer Progression by Inhibiting Wnt/β-Catenin Signaling and Promoting Apoptosis

doi: 10.3390/foods15091608

Figure Lengend Snippet: 10-HDA inhibited tumor growth and modified the histopathological features of HCT 116 xenografts in vivo. ( A ) Schematic overview of HCT 116 xenograft establishment and 10-HDA administration. ( B ) Representative images of xenograft tumors from saline-treated control mice and 10-HDA-treated mice, with corresponding tumor volume quantification. Control, blue; 100 mg/kg 10-HDA, orange; 200 mg/kg 10-HDA, purple. ( C ) H&E-stained sections of xenograft tumors. Red arrows indicate tumor cells; yellow arrows denote necrotic regions; blue arrows indicate atypical (pathological) mitotic figures; and green arrows indicate inflammatory cell infiltration. ( D ) Ki-67 immunohistochemical staining of xenograft tumors from control and 10-HDA-treated mice. n = 6; * p < 0.05, ** p < 0.01, **** p < 0.0001; ns, no statistical significance. Control, saline-treated tumor-bearing mice.

Article Snippet: Human colorectal carcinoma cell lines HCT 116 (ATCC; CCL-247) and HT-29 (ATCC; HTB-38) were purchased from Zhong Qiao Xin Zhou Biotechnology Co., Ltd. (Shanghai, China).

Techniques: Modification, In Vivo, Saline, Control, Staining, Immunohistochemical staining

Journal: Foods

Article Title: 10-Hydroxy-2-decenoic Acid Suppresses Colorectal Cancer Progression by Inhibiting Wnt/β-Catenin Signaling and Promoting Apoptosis

doi: 10.3390/foods15091608

Figure Lengend Snippet: Evaluation of the safety profile of 10-HDA treatment. ( A ) Mice weight changes compared with normal mice. Normal, red; model, blue; 100 mg/kg 10-HDA, orange; 200 mg/kg 10-HDA, purple. ( B ) Serum levels of liver function markers (AST, ALT, and ALP). ( C ) Representative H&E-stained histopathological sections of the heart, liver, spleen, lung and kidney. All analyses were performed on samples from healthy mice (normal control), and saline (model)- and 10-HDA-treated mice bearing HCT 116 xenograft tumors. n = 6; * p < 0.05, ** p < 0.01; ns, no statistical significance.

Article Snippet: Human colorectal carcinoma cell lines HCT 116 (ATCC; CCL-247) and HT-29 (ATCC; HTB-38) were purchased from Zhong Qiao Xin Zhou Biotechnology Co., Ltd. (Shanghai, China).

Techniques: Staining, Control, Saline

Transcriptomic analysis of ectopic xenograft tumor tissues derived from HCT 116 cells in 10-HDA- and saline-treated (control) mice. ( A ) Volcano plot of DEGs. Each dot represents one gene; red indicates significantly upregulated genes and green indicates significantly downregulated genes (FDR < 0.05 and |log2FoldChange| > 1). ( B ) Heatmap of representative DEGs related to tumor progression. Colors indicate relative expression (red, upregulation; blue, downregulation). ( C ) GO enrichment of DEGs showing top terms in biological process (BP, red), cellular component (CC, green), and molecular function (MF, blue). ( D ) Top 20 enriched KEGG pathways of DEGs. The x-axis shows the number of DEGs in each pathway; bar color represents statistical significance (adjusted p -value). ( E ) Relative mRNA expression levels of ANO1 , MKi67 , LTBP1 , FGF19 , NECTIN4 , PLD1 , and SKA1 in HCT 116 cells treated with 10-HDA or vehicle (control). Control, dark blue; 1.0 mM 10-HDA, blue; 1.5 mM 10-HDA, light blue; 2.0 mM 10-HDA, green. ( F ) Relative mRNA expression levels of ANO1 , MKi67 , LTBP1 , FGF19 , NECTIN4 , PLD1 , and SKA1 in xenograft tumors from 10-HDA- or saline-treated (control) mice. Control, green; 100 mg/kg 10-HDA, blue; 200 mg/kg 10-HDA, yellow. * p < 0.05, ** p < 0.01, **** p < 0.0001; ns, no statistical significance.

Journal: Foods

Article Title: 10-Hydroxy-2-decenoic Acid Suppresses Colorectal Cancer Progression by Inhibiting Wnt/β-Catenin Signaling and Promoting Apoptosis

doi: 10.3390/foods15091608

Figure Lengend Snippet: Transcriptomic analysis of ectopic xenograft tumor tissues derived from HCT 116 cells in 10-HDA- and saline-treated (control) mice. ( A ) Volcano plot of DEGs. Each dot represents one gene; red indicates significantly upregulated genes and green indicates significantly downregulated genes (FDR < 0.05 and |log2FoldChange| > 1). ( B ) Heatmap of representative DEGs related to tumor progression. Colors indicate relative expression (red, upregulation; blue, downregulation). ( C ) GO enrichment of DEGs showing top terms in biological process (BP, red), cellular component (CC, green), and molecular function (MF, blue). ( D ) Top 20 enriched KEGG pathways of DEGs. The x-axis shows the number of DEGs in each pathway; bar color represents statistical significance (adjusted p -value). ( E ) Relative mRNA expression levels of ANO1 , MKi67 , LTBP1 , FGF19 , NECTIN4 , PLD1 , and SKA1 in HCT 116 cells treated with 10-HDA or vehicle (control). Control, dark blue; 1.0 mM 10-HDA, blue; 1.5 mM 10-HDA, light blue; 2.0 mM 10-HDA, green. ( F ) Relative mRNA expression levels of ANO1 , MKi67 , LTBP1 , FGF19 , NECTIN4 , PLD1 , and SKA1 in xenograft tumors from 10-HDA- or saline-treated (control) mice. Control, green; 100 mg/kg 10-HDA, blue; 200 mg/kg 10-HDA, yellow. * p < 0.05, ** p < 0.01, **** p < 0.0001; ns, no statistical significance.

Article Snippet: Human colorectal carcinoma cell lines HCT 116 (ATCC; CCL-247) and HT-29 (ATCC; HTB-38) were purchased from Zhong Qiao Xin Zhou Biotechnology Co., Ltd. (Shanghai, China).

Techniques: Derivative Assay, Saline, Control, Expressing

10-HDA induced apoptosis in HCT 116 cells and subcutaneous HCT 116 xenograft tumors. ( A ) Relative mRNA expression levels of Bcl-2 , BAX , BINP2 , and caspase-3 in HCT 116 cells treated with 10-HDA or vehicle (control). Control, dark blue, 1.0 mM 10-HDA, blue; 1.5 mM 10-HDA, light blue; 2.0 mM 10-HDA, green. ( B ) Relative mRNA expression levels of Bcl-2 , BAX , BINP2 , and caspase-3 in xenograft tumors from 10-HDA- or saline-treated (control) mice. Control, green; 100 mg/kg 10-HDA, blue; 200 mg/kg 10-HDA, yellow. ( C ) Western blotting analysis of caspase-3, Bcl-2, BAX, and cleaved caspase-3 protein levels, as well as the calculated cleaved caspase-3/caspase-3 and Bcl-2/BAX ratios in HCT 116 cells treated with 10-HDA or vehicle (control). ( D ) Immunohistochemical staining of Bcl-2 and BAX in xenograft tumors from 10-HDA- or saline-treated (control) mice. * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001; ns, no statistical significance.

Journal: Foods

Article Title: 10-Hydroxy-2-decenoic Acid Suppresses Colorectal Cancer Progression by Inhibiting Wnt/β-Catenin Signaling and Promoting Apoptosis

doi: 10.3390/foods15091608

Figure Lengend Snippet: 10-HDA induced apoptosis in HCT 116 cells and subcutaneous HCT 116 xenograft tumors. ( A ) Relative mRNA expression levels of Bcl-2 , BAX , BINP2 , and caspase-3 in HCT 116 cells treated with 10-HDA or vehicle (control). Control, dark blue, 1.0 mM 10-HDA, blue; 1.5 mM 10-HDA, light blue; 2.0 mM 10-HDA, green. ( B ) Relative mRNA expression levels of Bcl-2 , BAX , BINP2 , and caspase-3 in xenograft tumors from 10-HDA- or saline-treated (control) mice. Control, green; 100 mg/kg 10-HDA, blue; 200 mg/kg 10-HDA, yellow. ( C ) Western blotting analysis of caspase-3, Bcl-2, BAX, and cleaved caspase-3 protein levels, as well as the calculated cleaved caspase-3/caspase-3 and Bcl-2/BAX ratios in HCT 116 cells treated with 10-HDA or vehicle (control). ( D ) Immunohistochemical staining of Bcl-2 and BAX in xenograft tumors from 10-HDA- or saline-treated (control) mice. * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001; ns, no statistical significance.

Article Snippet: Human colorectal carcinoma cell lines HCT 116 (ATCC; CCL-247) and HT-29 (ATCC; HTB-38) were purchased from Zhong Qiao Xin Zhou Biotechnology Co., Ltd. (Shanghai, China).

Techniques: Expressing, Control, Saline, Western Blot, Immunohistochemical staining, Staining

Involvement of Wnt/β-Catenin signaling in the anti-CRC effects of 10-HDA. ( A ) Relative mRNA expression levels of WNT11 , RNF43 , FRAT1 , β-catenin , and MMP7 in HCT 116 cells treated with 10-HDA or vehicle (control). Control, dark blue; 1.0 mM 10-HDA, blue, 1.5 mM 10-HDA, light blue; 2.0 mM 10-HDA, green. ( B ) Relative mRNA expression levels of WNT11 , RNF43 , FRAT1 , β-catenin , and MMP7 in xenograft tumors from 10-HDA- or saline-treated (control) mice. Control, green; 100 mg/kg 10-HDA, blue; 200 mg/kg 10-HDA, yellow. ( C ) Western blotting analysis of β-catenin expression in HCT 116 cells treated with 10-HDA or vehicle (control). ( D ) Western blotting analysis of β-catenin, p-GSK3β and GSK3β in xenograft tumor tissues from 10-HDA- and saline-treated (control) mice. ( E ) Immunohistochemical staining of β-catenin in xenograft tumors. ( F ) Western blotting analysis and densitometric quantification of the nuclear and cytoplasmic β-catenin in HCT 116 cells after 10-HDA or vehicle (control) treatment. * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001; ns, no statistical significance.

Journal: Foods

Article Title: 10-Hydroxy-2-decenoic Acid Suppresses Colorectal Cancer Progression by Inhibiting Wnt/β-Catenin Signaling and Promoting Apoptosis

doi: 10.3390/foods15091608

Figure Lengend Snippet: Involvement of Wnt/β-Catenin signaling in the anti-CRC effects of 10-HDA. ( A ) Relative mRNA expression levels of WNT11 , RNF43 , FRAT1 , β-catenin , and MMP7 in HCT 116 cells treated with 10-HDA or vehicle (control). Control, dark blue; 1.0 mM 10-HDA, blue, 1.5 mM 10-HDA, light blue; 2.0 mM 10-HDA, green. ( B ) Relative mRNA expression levels of WNT11 , RNF43 , FRAT1 , β-catenin , and MMP7 in xenograft tumors from 10-HDA- or saline-treated (control) mice. Control, green; 100 mg/kg 10-HDA, blue; 200 mg/kg 10-HDA, yellow. ( C ) Western blotting analysis of β-catenin expression in HCT 116 cells treated with 10-HDA or vehicle (control). ( D ) Western blotting analysis of β-catenin, p-GSK3β and GSK3β in xenograft tumor tissues from 10-HDA- and saline-treated (control) mice. ( E ) Immunohistochemical staining of β-catenin in xenograft tumors. ( F ) Western blotting analysis and densitometric quantification of the nuclear and cytoplasmic β-catenin in HCT 116 cells after 10-HDA or vehicle (control) treatment. * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001; ns, no statistical significance.

Article Snippet: Human colorectal carcinoma cell lines HCT 116 (ATCC; CCL-247) and HT-29 (ATCC; HTB-38) were purchased from Zhong Qiao Xin Zhou Biotechnology Co., Ltd. (Shanghai, China).

Techniques: Expressing, Control, Saline, Western Blot, Immunohistochemical staining, Staining

EdU displays enhanced synergy with CF10 due to increased incorporation into DNA. ( A ) Schematic demonstrating structures of CF10 and EdU with EdUTP and FdUTP incorporation causing DNA damage. The polyethylene glycol residue at the 5′ end of CF10 is highlighted within a red dotted oval and the arabinosyl cytosine (AraC) residue at the 3′ end is marked within the red oval. Single dose response in HCT116 cells (72 h) for ( B ) EdU, 5FU, and ( C ) CF10. Synergy distribution matrices for HCT116 cells treated with ( D ) EdU + 5FU and ( E ) EdU + CF10, obtained by COMBENEFIT software using HSA model. The EdU + CF10 combinations with highest synergy scores (SD-1: 2.5 µM EdU + 0.0156 µM CF10, SD-2: 2.5 µM EdU + 0.03125 µM CF10) are highlighted with a red box. ( F ) Confocal microscopy images of HCT116 cells treated with SD-1 and SD-2 along with the corresponding single doses of EdU (2.5 µM) and CF10 (CF10-1: 0.0156 µM and CF10-2: 0.03125 µM) for 48 h. NT was treated with fresh media in similar conditions. Treatment was followed by in situ click reaction with Cy5.5-azide to detect EdU. The cytoplasm was marked with β-actin (Alexa-488, green) and nuclei were stained with DAPI (blue). ( G ) Quantification of mean fluorescence intensity of EdU-Cy5.5 in panel (F) showing significantly increased incorporation of EdU in DNA in SD-1 and SD-2 compared to single-agent EdU. All data were represented as mean ± SD of minimum of 3 independent experiments, having 3 replicates in each set (**** P < .0001).

Journal: NAR Molecular Medicine

Article Title: The fluoropyrimidine polymer CF10 synergizes with 5-ethynyl-2′-deoxyuridine by promoting telomere attrition and mitotic catastrophe

doi: 10.1093/narmme/ugag005

Figure Lengend Snippet: EdU displays enhanced synergy with CF10 due to increased incorporation into DNA. ( A ) Schematic demonstrating structures of CF10 and EdU with EdUTP and FdUTP incorporation causing DNA damage. The polyethylene glycol residue at the 5′ end of CF10 is highlighted within a red dotted oval and the arabinosyl cytosine (AraC) residue at the 3′ end is marked within the red oval. Single dose response in HCT116 cells (72 h) for ( B ) EdU, 5FU, and ( C ) CF10. Synergy distribution matrices for HCT116 cells treated with ( D ) EdU + 5FU and ( E ) EdU + CF10, obtained by COMBENEFIT software using HSA model. The EdU + CF10 combinations with highest synergy scores (SD-1: 2.5 µM EdU + 0.0156 µM CF10, SD-2: 2.5 µM EdU + 0.03125 µM CF10) are highlighted with a red box. ( F ) Confocal microscopy images of HCT116 cells treated with SD-1 and SD-2 along with the corresponding single doses of EdU (2.5 µM) and CF10 (CF10-1: 0.0156 µM and CF10-2: 0.03125 µM) for 48 h. NT was treated with fresh media in similar conditions. Treatment was followed by in situ click reaction with Cy5.5-azide to detect EdU. The cytoplasm was marked with β-actin (Alexa-488, green) and nuclei were stained with DAPI (blue). ( G ) Quantification of mean fluorescence intensity of EdU-Cy5.5 in panel (F) showing significantly increased incorporation of EdU in DNA in SD-1 and SD-2 compared to single-agent EdU. All data were represented as mean ± SD of minimum of 3 independent experiments, having 3 replicates in each set (**** P < .0001).

Article Snippet: Human colorectal carcinoma cell line HCT116, human colonic adenocarcinoma cell line LS174T, and normal human intestinal epithelial cell line HIEC-6 were obtained from ATCC (ATCC numbers: CCL-247, CL-188, and CRL-3266, respectively).

Techniques: Residue, Software, Confocal Microscopy, In Situ, Staining, Fluorescence

EdU + CF10 demonstrates S-G2/M cell cycle arrest resulting from severe DNA damage in HCT116 cells. ( A ) Stacked bar diagram showing cell-cycle distribution of HCT116 cells 48 h post-treatment. ( B ) Dot plot representations showing total DNA content from propidium iodide staining ( x -axis) and γ-H2AX staining ( y -axis) with indicated treatments for 48 h. ( C ) Quantification of total γ-H2AX + cells for each treatment in panel (B). ( D ) Representative neutral comet assay images with the indicated treatment. As a positive control for DNA damage, HCT116 cells were treated with 0.1% of H 2 O 2 for 1 h. Scale bar is 50 μm. ( E ) Corresponding bar graph representations of the tail length obtained from the neutral comet assays with synergistic combinations. Data were represented as mean ± SD of minimum 3 independent experiments. ( F ) Confocal microscopy images detecting Top1cc with an antibody (Alexa-647, red) specific for the cleavage complex with the indicated treatments. Nuclei were stained with DAPI (blue). Nuclei with Top1cc foci are marked with white arrows in the corresponding images. Magnification was 100× with oil immersion. Scale bars are 15 μm. ( G ) Quantification of Top1cc foci per cell from images in panel (F). ( H ) Western blots of HCT116 cell lysates from indicated treatments for 48 h. Total cell lysates were probed with antibodies specific for phosphorylated Chk1 (pChk1), total Chk1, phosphorylated Chk2 (pChk2), total Chk2, and β-actin. For all results, values are depicted as mean ± SD of three independent data sets, having 3 replicates in each set. (* P < .05, ** P < .005, *** P < .001, **** P < .0001).

Journal: NAR Molecular Medicine

Article Title: The fluoropyrimidine polymer CF10 synergizes with 5-ethynyl-2′-deoxyuridine by promoting telomere attrition and mitotic catastrophe

doi: 10.1093/narmme/ugag005

Figure Lengend Snippet: EdU + CF10 demonstrates S-G2/M cell cycle arrest resulting from severe DNA damage in HCT116 cells. ( A ) Stacked bar diagram showing cell-cycle distribution of HCT116 cells 48 h post-treatment. ( B ) Dot plot representations showing total DNA content from propidium iodide staining ( x -axis) and γ-H2AX staining ( y -axis) with indicated treatments for 48 h. ( C ) Quantification of total γ-H2AX + cells for each treatment in panel (B). ( D ) Representative neutral comet assay images with the indicated treatment. As a positive control for DNA damage, HCT116 cells were treated with 0.1% of H 2 O 2 for 1 h. Scale bar is 50 μm. ( E ) Corresponding bar graph representations of the tail length obtained from the neutral comet assays with synergistic combinations. Data were represented as mean ± SD of minimum 3 independent experiments. ( F ) Confocal microscopy images detecting Top1cc with an antibody (Alexa-647, red) specific for the cleavage complex with the indicated treatments. Nuclei were stained with DAPI (blue). Nuclei with Top1cc foci are marked with white arrows in the corresponding images. Magnification was 100× with oil immersion. Scale bars are 15 μm. ( G ) Quantification of Top1cc foci per cell from images in panel (F). ( H ) Western blots of HCT116 cell lysates from indicated treatments for 48 h. Total cell lysates were probed with antibodies specific for phosphorylated Chk1 (pChk1), total Chk1, phosphorylated Chk2 (pChk2), total Chk2, and β-actin. For all results, values are depicted as mean ± SD of three independent data sets, having 3 replicates in each set. (* P < .05, ** P < .005, *** P < .001, **** P < .0001).

Techniques: Staining, Neutral Comet Assay, Positive Control, Confocal Microscopy, Western Blot

Synergistic combination of EdU + CF10 causes mitotic catastrophe in HCT116 cells. ( A ) Dot plot representations of flow cytometric analyses of pH3 levels with the indicated treatments for 48 h. Total DNA content was detected with propidium iodide ( x -axis) with anti-pH3 staining ( y -axis). ( B ) Quantification of phospho H3 + cells for treatments in panel (A). Single-agent EdU showed increased phospho H3 + cell population only in G2/M cells, whereas single-agent CF10, SD-1, and SD-2 showed significant phospho H3 positive cells in both G1 and S phase, consistent with premature chromatin condensation. ( C ) Confocal micrographs of representative spindle fiber assembly images from each treatment group with microtubules in spindle fibers stained with antibody specific for α-tubulin, conjugated with Alexa-647 fluorophore (red). The pericentromeric regions of the chromosomes were stained with antibody targeting pH3, conjugated with Alexa-488 (green). Nuclei were stained with DAPI (blue). A monopolar spindle fiber is shown from a representative SD-1 cell, while representative images from SD-2 treated cells highlight a multipolar spindle fiber assembly. Only in cells treated with SD-1 or SD-2 was abnormal spindle fiber formation detected confirming mitotic catastrophe. Magnification was 100× with oil immersion; scale bar is 10 μm. ( D ) Dot plots obtained from the flow cytometric analyses of Annexin V/PI assay from cells treated with the indicated treatments. ( E ) Quantification of Annexin V/PI assay results from panel (D). ( F ) Graphical representation of caspase-3/7 Glo assay on cells with the indicated treatments. Results suggest HCT116 cells undergo mitotic catastrophe with activation of apoptosis with SD-1 and SD-2 treatment. Data were represented as mean ± SD of minimum 3 independent experiments, having 3 replicates in each set (* P < .05, ** P < .005, *** P < .001, **** P < .0001).

Journal: NAR Molecular Medicine

Article Title: The fluoropyrimidine polymer CF10 synergizes with 5-ethynyl-2′-deoxyuridine by promoting telomere attrition and mitotic catastrophe

doi: 10.1093/narmme/ugag005

Figure Lengend Snippet: Synergistic combination of EdU + CF10 causes mitotic catastrophe in HCT116 cells. ( A ) Dot plot representations of flow cytometric analyses of pH3 levels with the indicated treatments for 48 h. Total DNA content was detected with propidium iodide ( x -axis) with anti-pH3 staining ( y -axis). ( B ) Quantification of phospho H3 + cells for treatments in panel (A). Single-agent EdU showed increased phospho H3 + cell population only in G2/M cells, whereas single-agent CF10, SD-1, and SD-2 showed significant phospho H3 positive cells in both G1 and S phase, consistent with premature chromatin condensation. ( C ) Confocal micrographs of representative spindle fiber assembly images from each treatment group with microtubules in spindle fibers stained with antibody specific for α-tubulin, conjugated with Alexa-647 fluorophore (red). The pericentromeric regions of the chromosomes were stained with antibody targeting pH3, conjugated with Alexa-488 (green). Nuclei were stained with DAPI (blue). A monopolar spindle fiber is shown from a representative SD-1 cell, while representative images from SD-2 treated cells highlight a multipolar spindle fiber assembly. Only in cells treated with SD-1 or SD-2 was abnormal spindle fiber formation detected confirming mitotic catastrophe. Magnification was 100× with oil immersion; scale bar is 10 μm. ( D ) Dot plots obtained from the flow cytometric analyses of Annexin V/PI assay from cells treated with the indicated treatments. ( E ) Quantification of Annexin V/PI assay results from panel (D). ( F ) Graphical representation of caspase-3/7 Glo assay on cells with the indicated treatments. Results suggest HCT116 cells undergo mitotic catastrophe with activation of apoptosis with SD-1 and SD-2 treatment. Data were represented as mean ± SD of minimum 3 independent experiments, having 3 replicates in each set (* P < .05, ** P < .005, *** P < .001, **** P < .0001).

Techniques: Staining, Glo Assay, Activation Assay

EdU + CF10 synergistic combinations cause telomere attrition in HCT116 cells. ( A ) IF-FISH analysis of representative cells from each treatment group, 48 h post-treatment. A negative control was maintained by treating the cells with telomerase inhibitor BIBR1532 (10 μM, 24 h). The telomeres were marked using a PNA TelC probe conjugated with Alexa-488 fluorophore (green). Replication protein A 32 (RPA32) foci formation was detected using an RPA32-specific Ab conjugated with Alexa-647 (red). Nuclei were stained with DAPI (blue). The magnification was 100× with oil immersion; scale bar is 5 μm. Quantification of ( B ) telomere staining per cell and ( C ) RPA32 foci per cell. Data show significant reduction in telomere signal and significant increase in RPA32 foci with SD-1 and SD-2. ( D ) Q-FISH micrographs of metaphase spread prepared from synchronized HCT116 cells with the indicated treatments for 48 h. Prior to drug treatment, cells were synchronized in S phase using a double thymidine block. Cells were incubated in microtubule inhibitor colcemid (0.1 μg/ml) for 12 h post-treatment to prepare a metaphase spread. Telomere regions on the chromosomes were probed with PNA TelC-Alexa-488 (green). Chromosomes were stained with DAPI (blue). Magnification was 100× with oil immersion. Scale bar is 15 μm. ( E ) Corresponding violin plot of telomere fluorescence unit (TFU) in each treatment group, measured using TFL-Telo software, considering 1 TFU as 1 kb. Results are depicted as mean ± SD of three independent data sets, having 3 replicates in each set (* P < .05, ** P < .005, *** P < .001, **** P < .0001).

Journal: NAR Molecular Medicine

Article Title: The fluoropyrimidine polymer CF10 synergizes with 5-ethynyl-2′-deoxyuridine by promoting telomere attrition and mitotic catastrophe

doi: 10.1093/narmme/ugag005

Figure Lengend Snippet: EdU + CF10 synergistic combinations cause telomere attrition in HCT116 cells. ( A ) IF-FISH analysis of representative cells from each treatment group, 48 h post-treatment. A negative control was maintained by treating the cells with telomerase inhibitor BIBR1532 (10 μM, 24 h). The telomeres were marked using a PNA TelC probe conjugated with Alexa-488 fluorophore (green). Replication protein A 32 (RPA32) foci formation was detected using an RPA32-specific Ab conjugated with Alexa-647 (red). Nuclei were stained with DAPI (blue). The magnification was 100× with oil immersion; scale bar is 5 μm. Quantification of ( B ) telomere staining per cell and ( C ) RPA32 foci per cell. Data show significant reduction in telomere signal and significant increase in RPA32 foci with SD-1 and SD-2. ( D ) Q-FISH micrographs of metaphase spread prepared from synchronized HCT116 cells with the indicated treatments for 48 h. Prior to drug treatment, cells were synchronized in S phase using a double thymidine block. Cells were incubated in microtubule inhibitor colcemid (0.1 μg/ml) for 12 h post-treatment to prepare a metaphase spread. Telomere regions on the chromosomes were probed with PNA TelC-Alexa-488 (green). Chromosomes were stained with DAPI (blue). Magnification was 100× with oil immersion. Scale bar is 15 μm. ( E ) Corresponding violin plot of telomere fluorescence unit (TFU) in each treatment group, measured using TFL-Telo software, considering 1 TFU as 1 kb. Results are depicted as mean ± SD of three independent data sets, having 3 replicates in each set (* P < .05, ** P < .005, *** P < .001, **** P < .0001).

Techniques: Negative Control, Staining, Blocking Assay, Incubation, Fluorescence, Software

Review

human colorectal carcinoma line hct 116 (ATCC)

Structured Review

Images

1) Product Images from "Integrated transcriptomic and proteomic validation identifies SLC35D3 as a tumor-selective surface antigen for colorectal and neuroendocrine carcinomas"

Related Articles

Immunohistochemical staining:

Expressing:

Positive Control:

Staining:

Negative Control:

Over Expression:

Biomarker Discovery:

Flow Cytometry:

Comparison:

Similar Products

Image Search Results